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seudomonas aeruginosa (P. aeruginosa) have a considerable risk to public health in the world, due to its high ability to develop resistance to different classes of antibiotics. It has been discovered as a prevalent coinfection pathogen that causes sickness exacerbation in COVID-19 patients. This study aimed to determine the prevalence of P. aeruginosa from COVID-19 patients in Al Diwaniyah province, Iraq and to identify its genetic resistance pattern. 70 clinical samples were obtained from severe cases of patients (RT-PCR positive for SARS-COV-2 on a nasopharyngeal swab) who attended Al Diwaniyah Academic Hospital. 50 P. aeruginosa bacterial isolates were detected via microscopic examination, routine cultured and biochemical testing, then validated by the VITEK-2 compact system. VITEK reported 30 positive results, which later confirmed through molecular detection using 16s RNA specific for detection and a phylogenetic tree.20 isolates had positive PCR findings and 5 isolates submitted to GenBank with accession numbers OL314557.1, OL314556.1, OL314555.1, OL314554.1, OL314553.1.For antibiotic resistance genes, the number of the isolates containing blaOXA-1 and blaCTX-M 18 (90 percent) and 16 (80 percent) respectively. To study its adaptation in a SARS-CoV-2 infected environment, genomic sequencing investigations were undertaken with phenotypic validation. In conclusion, we demonstrate that multidrug resistant P. aeruginosa play an important role in in vivo colonization in COVID-19 patients and could be one of the causes of death of these patients which indicates the great challenge to clinicians in the facing of this serious disease.

This study aimed to examine Interleukin-6 (IL-6) as predictive biomarker for COVID-19 severity, to achieve this aim, the protein concentration degree and gene expression was tested in 90 samples according to COVID-19 severity which had been classified as (Control, Mild, Moderate, and Severe COVID-19). The IL-6 gene expression was assessed by means of qPCR and the protein concentration changed into analyzed via ELIZA and Western Blot. The consequences revealed that, there was a positive correlation amongst IL-6 gene and protein levels to severity of COVID-19. Accordingly, the IL-6 play essential role as predictive biomarker for COVID-19 severity and play a pivotal function in COVID-19 prediction. Additional studies are wanted to verify these findings in larger cohorts and to analyze the precise mechanisms of IL-6 gene regulation across the various stages of COVID-19 infections.

Sleep issues after recovery have gotten a limited amount of investigation, despite the fact that the effects of COVID-19 are becoming more commonly understood. Students which had made a full recovery from COVID-19 were compared to individuals who had never been infected in order to determine the links between the between sleep interruptions and students who had recovered. In this research, both students evaluations were conducted on pupils who had recovered from COVID-19 as well as individuals who had never been infected again. The students were paired using age, gender, education and socioeconomic background. The study had a total of 110 participating students. In-between November of 2021 and March of 2022, students from the Pharmacy College and other colleges belonging to Al-Qadisiyah University were selected. A socio-demographic and clinical checklist was used together with the Pittsburgh Sleep Quality Index and the Hospital Anxiety Depression Scale in the interviews done with participants. Other measures used in this study include the Pittsburgh Sleep Quality Index and the Hospital Anxiety Depression Scale. However, fifty percent of those who recovered reported sleep problems whereas fifty percent of those that were nonrecoverable cases did not. A total of fifty-five percent (55/1110) of recovered COVID-19 students and one hundred percent (55/55) of control participants were found to have sleep disturbances, respectively. The recovered participants, with the exception of those who used medications, exhibited significantly higher levels of sleep instabilities across all domains that were measured by the Pittsburgh Sleep Quality Index questionnaire. This group of domains comprised There was a significant difference (P < 0.001) in the quality of sleep, latency, duration, efficiency, disruptions, and daytime dysfunction, as well as the overall PSQI score (P ≤ 0.001). First, it was demonstrated that students suffering from covid 19 recovered have a worse subjective sleep quality and that major depressive symptoms did not contribute significantly to sleep measure and numerous researchers have agreed on the need for further researches in order to uncover the complex relationship between Covid 19 and sleep. 2.3%, confidence interval with a 95% rate: Some typical symptoms of the sleep disorder include are measures of sleep quality and some indicators of the hyperhidrosis and abnormal pandemic waters of the covid-19. The Covid-19’s relationship to sleep difficulties is still a researchable area of concern as a possible consequence of COVID-19. This is because COVID-19 has the capacity to affect either the physical or the psychic that leads to the impairment of either psychic or physical functions.

Summary This is the second research was take place to microbiological and physiological evaluations have shown that Exam anxiety (EAE) can alter the health of stomach of students during exam and this lead to infected with Helicobacter pylori was known as a pathogen related to peptic ulcers and gastric carcinoma . We observed into whether pharmacy students through exams are stressful enough to cause changes in gastric secretion as consequences Helicobacter pylori infected. Procedures: This study designed to evaluate H. pylori infection in student during exam had morning sickness nausea and vomiting related to the anxiety and gastric secretion. casual sample of female and male second stages of students from college of Pharmacy in Al-Qadisiyah University carried to inspect were taken before second final-course exam and during the exam of mid-course determination(1 month). Finally, from 137 students 87 student were infected (20-21years) , with sever to moderate nausea attended the outpatient and specialized clinic. The serological frequency of H. pylori was 63.5% in students, compare with non-infected students 36.5% of the cases who had mild gastric pain, nausea, and burning of the stomach due to anxiety from exam. Most infected student with H. pylori were the severe symptoms (57.5%) moderate (28.%), and mild (20%) cases. This study found that severe nausea and vomiting, dyspepsia, and other gastrointestinal symptoms during exam were increased with the infection by H. pylori; and the same times may be gastric secretion increased significantly (p≤0.001) condition consequently, it is a danger factor for difficulties in student and its poor outcomes, especially in developing countries, such as Iraq. These results can be minimized by improving the socioeconomic and public health conditions. H. pylori infection in student is considered a main health problem and should be treated before . Further investigations are required in this regard and researchers are recommended to conduct studies on the Exam anxiety to recognize How it has the specific pathophysiology related to H. pylori infection.