Pseudomonas aeruginosa is an opportunistic clinical pathogen, and it's found in different organisms such as soil,
plants and water and considered common pathogenic infections that causes nosocomial infections Present study
included collecting about 316 random samples in different ways from January 2022 to February 2023 from Al
Diwaniyah teaching hospitals the combination process was randomly investigated for P. aeruginosa bacteria
specimens were inculcated on different culture media like Blood-agar, MacConkey-agar then Muller Hinton-agar to
find specimen of P. aeruginosa based on their phenotypic features, for the identification and resistance to antibiotics
study biochemical tests and Api 20 E system were used. 316 samples, 33(25.58%) P. aeruginosa were isolated from
ear, 16(21.33%) from burn, 8 (16%) from wound, 6 (14.28%) from urine and 2(10%) from sputum. In males
27(19.56%) isolate and 38(21.34%) in female. P. aeruginosa biochemical examinations showed that all specimens
produce catalase, oxidase, then growth at (42°C) and most of the specimens non able to yield (H2S), reduce nitrates
and hemolysis β-hemolytic. While they gave negative results for Methyl-red, Voges Proskauer and Indole test as
diagnosed with Api 20 E system to further confirm the isolation yield for P. aeruginosa bacteria. Majority of isolates
55(84.61%) showed in results had Ceftriaxone resistance, furthermore other specimen showed different antibiotics
sensitivity response. Isolation proportion of P. aeruginosa from ear is more than the isolation rate from other body
sites in patients. Nearly every P. aeruginosa specimen showed resistance to most antibiotics in present study,
particularly Ceftriaxone where resistance rate was 55 (84.61%).
